Instructions - Quick Start

1.  Turn on the LED light.

2.  Apply the sample

All it takes is a very small amount of fluid.  The field of view is about the size of a grain of sand so it does not take much, but it must be on the center.  If you want to look at a dry sample it should be immersed in water or another fluid first.

3.  View

For general viewing just look through the eyepiece or mount a camera on the scope.  We recommend starting out by viewing through the eyepiece.  After you have confirmed that you have an interesting sample of fluid it is easier to mount a camera.  You can push the sample around on the lens surface with the tip of the pipette to reposition your sample if nothing of interest is visible in the field of view.

Instructions - Tips, Tricks, and Details

Attach the iPhone Mount and Align the Camera

 

Slip the mount over the eyepiece and push it on until it firmly meets the top of the eyepiece.  If the mount is not fully seated on the eyepiece the camera will not be close enough to the eyepiece.  Similarly, some device cases are too thick to allow the camera lens to get close enough to the eyepiece.  The brace under the mount should line up directly over the center of the scope.  This position balances the phone over the center of the scope so that it is less likely to tip over when the phone is placed on the mount.

The time to align the camera is when the camera and the scope are both on.  Start with the camera above the scope and center the eyepiece in the view.  As you lower the camera onto the mount keep the eyepiece in the center of the view.  As you get closer the light will be visible, and try to keep the brightest light in the center.  This step can be a little tricky because the camera must be aligned precisely over the center of the eyepiece.  When the phone is aligned the camera will see what you see by eye through the eyepiece.  If you have difficulties the most common problem is that the final resting place of the camera is too far from the eyepiece because of the phone case.  Don't be afraid to experiment a little to get the best result.  After the camera is mounted you can use the camera zoom to fill the frame.  Most phones perform best in video mode since the video camera zooms in closer than the wider angle picture mode.

Adjust the Depth of Focus and Resolution with the Diffuser

 

If want to increase the resolution and reduce the depth of focus insert the included diffuser between the LED light source and the objective lens where you have placed the sample.  Raising the diffuser so that it touches the LED is the best starting position.  Lowering the diffuser until it almost touches the sample will increase resolution but also decrease the depth of focus.

Use of Plastic Cover Slips

 

Cover slips can be used to thin your sample and keep it from drying.  This is helpful if the sample is blood.  Take care not to let the sample dry completely and glue the cover slip to the objective lens.  The lens may be damaged if the cover slip is left to dry on the lens.

Replace Batteries

 

Warning:  The battery compartment can only be removed and replaced if the power switch is in the "on" position.  It was designed this way so that the battery compartment will not fall out when the scope is in "off" position.  Turn the scope "on" and push up on the switch with a finger nail until the battery compartment is up far enough to grab from the top.  The wires remain attached during battery replacement.  Open the compartment by pushing on the raised ribs with your thumb and the cover will slide off.  Take care not to twist the wires when replacing the battery compartment gently.  Make sure the wires are not pinched, twisted or hung up.

Clean up

 

Please gently clean the scope with a slightly damp cloth taking care not to wipe the lens too roughly.  .  Window cleaning fluid is the best.  We use Windex.  Spray the liquid on the cloth and not directly on the scope.  The lens cleaning tissues we included are only included for your convenience.  Any absorbent cloth will work, but be gentile with the objective lens.  It is somewhat delicate especially where the adhesive holds the small disk of glass to the metal objective lens case.

Warning:  The scope is not waterproof and will be damaged by submersion.

4D Optical LLC

MicrobeScope

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